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. 2023 Oct 24;12(21):2514. doi: 10.3390/cells12212514

Figure 3.

Figure 3

Gene expression profiles in 2D- and 3D-cultured cells. The four cell types were cultured for three days in 96-well plates without or with Matrigel for 2D or 3D cultures, respectively. Gene expression was analyzed using qPCR. (A) Hepatocyte-related gene expression profiles in 2D- and 3D-cultured cells. (B) Inflammation-related gene expression in 2D- and 3D-cultured cells. (C) Extracellular-matrix-related gene expression in 2D- and 3D-cultured cells. (D) Endothelial-cell-related gene expression in 2D- and 3D-cultured cells. Target gene expression was normalized using GAPDH expression. Relative fold changes in mRNA expression were measured using the 2−(ΔΔCt) method. The results are presented as the means ± standard deviation (SD) of three replicates. * p < 0.05, ** p < 0.01, and *** p < 0.001. 2D; two-dimensional-cultured cells, Orga; 3D-cultured liver organoid.