Table 4.
Isobolographic analysis of interactions between MGN and DCT (at the fixed ratio of 1:1) for nonparallel concentration–response effects in various GC cell lines.
| Cell Line | IC50exp (nexp) (µg/mL) |
Lower IC50add (nadd) (µg/mL) |
Upper IC50add (nadd) (µg/mL) |
t-Test |
|---|---|---|---|---|
| ACC-201 | 5.70 ± 1.04 (72) | 6.60 ± 1.16 (176) | 9.70 ± 1.21 (176) | t = 0.578; df = 219.6 p = 0.564 |
| AGS | 6.86 ± 1.22 (72) | 6.63 ± 1.61 (176) | 10.56 ± 1.70 (176) | t = 0.114; df = 239.2 p = 0.909 |
| MKN-74 | 11.43 ± 2.34 (90) | 11.38 ± 4.03 (176) | 23.43 ± 4.11 (176) | t = 0.011; df = 255.7 p = 0.991 |
| NCI-N87 | 7.66 ± 2.19 (90) | 8.82 ± 3.41 (176) | 24.56 ± 3.82 (176) | t = 0.286; df = 261.6 p = 0.775 |
The IC50 values (in µg/mL ± SEM) for the mixture of MGN with DCT were determined experimentally (IC50exp) in the in vitro MTT assay. The IC50add values were calculated from the lower and upper isoboles of additivity; nmix: total number of items experimentally determined; nadd: total number of items calculated for the additive two-drug mixture.