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Gating strategy for evaluation of the number of AFU (active fluorescent units) and n-AFU (non-active fluorescent units) cells. Cells were displayed in forward scatter (FSC) vs. side scatter (SSC) plot in logarithmic scale, and a gate named L. plantarum 299 v is used to capture the cells of interest. Right dot plot shows gating strategy for live and dead L. plantarum 299 v analysis where SYTO24 and PI were employed.
