Figure 1.

Precise mapping of two conserved N-terminal regions of CP190 that interact with architectural proteins. (A) Schematic presentation of the CP190 domain structure. The scale rule (in aa) is shown at the top of the figure. The highly conserved regions for the CP190 protein among Drosophila species are marked with orange lines. (B) Mapping of CP190 domains interacting with Opbp, M1BP, and ZIPIC proteins in the Y2H assay. Variants of CP190 were fused to the GAL4 DNA-binding domain and tested for interaction with the C2H2 proteins fused to the GAL4 activating domain. The results are summarized in columns with “+” and “−” signs referring to the presence and absence of interaction, respectively. The growth assay yeast plates are shown in Supplementary Materials Figure S3.