TABLE 3.
Sizes of the restriction fragments common to all naphthalene-catabolic plasmids from site-derived isolates and P. putida NCIB9816-4a
| Size of fragment (kb) with restriction enzyme:
| ||||
|---|---|---|---|---|
| SmaI | BamHI | XhoI | HindIII | |
| >23.0b | >23.0b | >23.0b | 16.1c | 3.3 |
| 11.6 | 23.0 | 10.9 | 13.2 | 2.5 |
| 7.2 | 20.0 | 6.0 | 11.2 | 2.0d |
| 5.8 | 9.3 | 5.2 | 9.8 | 1.6 |
| 5.0 | 5.5 | 3.9 | 8.6 | 1.3 |
| 3.4 | 4.0 | 2.4 | 7.8 | 1.2 |
| 1.9 | 1.9 | 6.4c | 1.0 | |
| 1.5 | 4.5 | 0.9 | ||
| 1.4 | 3.4 | 0.8 | ||
a
See the text for minor variations from this pattern for certain plasmids.
b
Resolution of standard agarose gels (0.7%) did not allow accurate sizing of fragments greater than 23 kb.
c
Plasmids contain either the 16.1-kb fragment (Cg1, Cg4, Cg5, Cg7, Cg11, Cg15) or the 6.4-kb fragment (PpNCIB9816-4, Cg2, Cg8, Cg9, Cg12, Cg15, Cg21).
d
Doublet.