FIGURE 5.

A multi-method approach is necessary to elucidate the role of dynamic microtubule end conformations in microtubule stability. Light microscopy approaches provide both spatial and temporal information over large timescales. Electron microscopy approaches are used to gain high-spatial-resolution insights into fixed microtubule end conformations.^{[105,106,111]} Computational approaches bridge the molecular to polymer scales, allowing simulation of molecular interactions through space and time using a variety of assumptions and input parameters.^[109]