Table 3.
Chemicals | Supplier | Dilution | Catalog Number |
---|---|---|---|
Paraformaldehyde | Sigma-Aldrich, Inc., St. Louis, MO, USA | 4% | 158127 |
Phosphate-buffered saline (PBS) | Sigma-Aldrich, Inc., St. Louis, MO, USA. | manufacturer’s notice | P4417 |
Triton X100 | Sigma-Aldrich, Inc., St. Louis, MO, USA. | 0.1% | X100 |
0.2% | |||
Hydrogen Peroxide (H2O2) | Sigma-Aldrich, Inc., St. Louis, MO, USA. | 0.3% | 1085971000 |
Fetal Bovine Serum | Sigma-Aldrich, Inc., St. Louis, MO, USA. | 25% | F7524 |
0.1% | |||
DAB | Sigma-Aldrich, Inc., St. Louis, MO, USA. | manufacturer’s notice | D5905 |
Trizma® base | Sigma-Aldrich, Inc., St. Louis, MO, USA. | manufacturer’s notice | T1503 |
Hydrochloric acid (HCl) | Avantor delivered by VWR Radnor, PA, USA |
manufacturer’s notice | 35328 |
Masson trichrome with aniline blue staining | Bio-Optica Milano S.p.a Milan, Italy |
manufacturer’s notice | 04-010802 |
Sorensen’s Phosphate Buffer, 0.2 M | Avantor delivered by VWR Radnor, PA, USA. |
manufacturer’s notice | 100496-400 |
Glutaraldehyde | Sigma-Aldrich, Inc., St. Louis, MO, USA. | 2.5% | G5882 |
Fluoromount Aqueous Mounting Medium | Sigma-Aldrich, Inc., St. Louis, MO, USA. | manufacturer’s notice | F4680 |