Table 2.
Calculated hemolysis ratios according to Equation (1) for enalaprilat and enalaprilat-loaded CaP particles, HNPs, and CSNPs on 1% rabbit erythrocyte solution. The data were averaged from three experimental replicates, each containing two technical replicates, using 10% Triton X-100 and PBS samples as positive and negative controls, respectively.
| Samples | Chitosan Type | Calculated Hemolysis, % | |
|---|---|---|---|
| Undiluted | Dilution 1/5 | ||
| Enalaprilat | - | 0.42 ± 0.38 | 0.00 ± 0.12 |
| CaP | - | 2.02 ± 0.26 | 0.00 ± 0.46 |
| Hybrid | 5 kDa chitosan | 4.76 ± 0.47 | 0.00 ± 0.22 |
| 72 kDa glycol chitosan | 3.50 ± 0.44 | 0.00 ± 0.14 | |
| Chitosan | 5 kDa chitosan | 3.56 ± 0.36 | 0.00 ± 0.12 |
| 72 kDa glycol chitosan | 13.80 ± 1.14 | 0.00 ± 0.51 | |