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The coding regions of the BWC1 and BWC2 genes were fused adjacent to the AD or BD of S. cerevisiae GAL4. Plasmids were cotransformed into a S. cerevisiae strain in which Gal4 regulates the ADE2, HIS3, and lacZ genes. Growth of strains in the absence of adenine (−ade) or histidine (−his) and increased β-galactosidase activity (β-gal, ± one standard error, Miller units) indicate protein-protein interactions.
