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Ten mice each were infected intranasally with 1 × 105 cells of wild-type, bwc1 mutant, and bwc2 mutant, and reconstituted (bwc1 + BWC1; bwc2 + BWC2) serotype A strains, and survival monitored daily. Mice infected with the wild-type and complementing strains progress to severe morbidity at the same rate, whereas mice infected with the bwc1 or bwc2 mutant strains survived twice as long.
