FIG 3.

Comparison of five antimicrobial classes plus phage (EM or 14207) against I0003-1 and AR351. Average biofilm cell reduction seen with single phage plus antibiotic combinations against AR351 and I0003-1 in biofilm time-kill analyses of EM or 14207, respectively, with CIP, MEM, AZT, AMK, and COL at 0.25× MIC and a theoretical MOI of 1.0. Subinhibitory antibiotic concentrations are used to mimic the expected drug ineffectiveness that would otherwise not occur in this simplified in vitro system. The bars represent the difference in 24-h CFU/cm² from baseline. Values are means ± standard deviations from two biological replicates. 14207, 14207 phage; AMK, amikacin; AZT, aztreonam; CIP, ciprofloxacin; COL, colistin; EM, EM phage; MEM, meropenem; MIC, minimum inhibitory concentration; MOI, multiplicity of infection; TK, time-kill.