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. 2023 Oct 12;67(11):e00933-23. doi: 10.1128/aac.00933-23

TABLE 3.

Plasmids used in this study

Plasmid Description References
pAg1 A streamlined version of the binary vector pBIN19 containing sequences necessary for replication in E. coli and Agrobacterium tumefaciens (oriV and trfA), E. coli neomycin phosphotransferase gene (nptII), and the transferable DNA (T-DNA) region, with a multiple cloning site within the T-DNA region (54)
pAg1-TinCyp51B/T The 5′ UTR of the TinCYP51B gene (2.52 kb; GenBank accession no. OK539858), the nptII cassette [the promoter sequence of Aspergillus nidulans tryptophan C (trpC) gene (PtrpC; GenBank accession no. X02390), nptII, the termination sequence of Aspergillus fumigatus cgrA gene (TcgrA; GenBank accession no. EAL84894)], the 3′ UTR of the TinCYP51B gene (2.51 kb) (12)
pAg1-TinCYP51B/R The 5′ UTR and ORF of the TinCYP51B gene (4.32 kb), the nptII cassette (PtrpC, nptII, TcgrA), the 3′ UTR of the TinCYP51B gene (2.51 kb) This study