TABLE 2.
MICs of meropenem and cefepime alone and in combination with BLIs against the panel of engineered P. aeruginosa strains producing various cloned β-lactamases
| Strain | Β-lactamΒ-lactamase | Class | MEM | MEM + XER | MEMP + VAB | MEM + AVI | MEM + REL | MEM + TAN | MEM + DUR | FEP | FEP + XER | FEP + VAB | FEP + AVI | FEP + REL | FEP + TAN | FEP + DUR |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PAM4175 | none | 0.125 a | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | 0.25 | 0.125 | 0.25 | 0.125 | 0.25 | 0.125 | ≤0.06 | |
| PAM4743 | CTX-M-15 | A | 0.25 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | >64 | 0.125 | 1 | 0.125 | 1 | 0.25 | 0.125 |
| PAM4821 | CTX-M-25 | A | 0.5 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | 0.125 | >64 | 0.125 | 4 | 0.25 | 1 | 0.25 | 0.125 |
| PAM4823 | CTX-M-27 | A | 0.5 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | >64 | 0.125 | 2 | 0.25 | 2 | 0.25 | 0.125 |
| PAM4800 | GES-19 | A | 1 | ≤0.06 | 0.125 | ≤0.06 | 0.125 | ≤0.06 | ≤0.06 | 64 | 0.125 | 1 | 0.25 | 0.5 | 0.125 | ≤0.06 |
| PAM4801 | GES-20 | A | 8 | ≤0.06 | 0.25 | ≤0.06 | 0.25 | ≤0.06 | ≤0.06 | 2 | 0.125 | 0.25 | 0.25 | 0.25 | 0.25 | ≤0.06 |
| PAM4135 | KPC-2 | A | 32 | ≤0.06 | ≤0.06 | ≤0.06 | 0.125 | ≤0.06 | ≤0.06 | >64 | 0.125 | 0.25 | 0.25 | 0.25 | 0.25 | 0.125 |
| PAM4689 | KPC-3 | A | 64 | ≤0.06 | ≤0.06 | ≤0.06 | 0.125 | ≤0.06 | ≤0.06 | >64 | 0.125 | 0.25 | 0.25 | 0.5 | 0.25 | 0.25 |
| PAM4825 | MIR-1 | C | 0.5 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ND | 2 | ≤0.06 | 0.125 | 0.125 | 0.125 | 0.125 | ND |
| PAM4840 | OXY-6–2 | A | 0.25 | ≤0.06 | 0.125 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | 8 | 0.125 | 4 | 0.25 | 0.25 | 0.25 | 0.125 |
| PAM4842 | PER-2 | A | 0.125 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | 64 | 0.125 | 0.5 | 0.25 | 0.25 | 0.125 | ≤0.06 |
| PAM4907 | PER-4 | A | 0.125 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | 64 | 0.125 | 16 | 32 | 32 | 0.5 | 0.5 |
| PAM4874 | SHV-12 | A | 0.25 | ≤0.06 | 0.125 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | 64 | 0.125 | 4 | 0.25 | 1 | 0.25 | ≤0.06 |
| PAM4744 | SME-2 | A | 8 | ≤0.06 | ≤0.06 | ≤0.06 | 0.25 | ≤0.06 | 0.125 | 0.5 | ≤0.06 | 0.125 | 0.125 | 0.125 | 0.25 | 0.125 |
| PAM4878 | TEM-10 | A | 0.125 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | 16 | ≤0.06 | 2 | 0.125 | 0.25 | 0.25 | ≤0.06 |
| PAM4908 | VEB-1 | A | 0.5 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | >64 | 0.125 | 2 | 0.25 | 0.25 | 0.25 | ≤0.06 |
| PAM4910 | VEB-2 | A | 0.25 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | >64 | 0.125 | 4 | 0.25 | 0.5 | 0.5 | ≤0.06 |
| PAM4186 | CMY-2 | C | 0.25 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ND | 1 | ≤0.06 | 0.125 | ≤0.06 | ≤0.06 | ≤0.06 | ND |
| PAM4745 | P99 | C | 1 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | 0.125 | 8 | 0.125 | 0.25 | 0.125 | 0.125 | 0.125 | ≤0.06 |
| PAM4869 | PDC-1 | C | 0.5 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | 4 | 0.125 | 0.5 | 0.25 | 0.125 | 0.125 | 0.125 |
| PAM4884 | ADC-181 | C | 0.125 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ND | 0.5 | ≤0.06 | 0.25 | ≤0.06 | ≤0.06 | 0.125 | ND |
| PAM4827 | OXA-1 | D | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | 1 | ≤0.06 | 1 | ≤0.06 | 0.25 | 0.25 | ≤0.06 |
| PAM4792 | OXA-2 | D | 2 | ≤0.06 | 2 | 0.5 | 0.5 | ≤0.06 | 0.125 | 0.5 | ≤0.06 | 0.5 | 0.125 | 0.125 | 0.125 | ≤0.06 |
| PAM4846 | OXA-9 | D | 0.25 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | ≤0.06 | 2 | 0.125 | 0.25 | 0.125 | 1 | 0.125 | 0.125 |
| PAM4217 | OXA-48 | D | 4 | ≤0.06 | 4 | ≤0.06 | 2 | 0.125 | 0.125 | 0.5 | 0.125 | 0.5 | 0.125 | 0.25 | 0.125 | 0.125 |
| PAM4875 | OXA-23 | D | 2 | ≤0.06 | 2 | 0.5 | 2 | 2 | ≤0.06 | 2 | ≤0.06 | 2 | 0.25 | 1 | 1 | ≤0.06 |
| PAM4876 | OXA-72 | D | 2 | ≤0.06 | 2 | 0.5 | 2 | 2 | ≤0.06 | 2 | ≤0.06 | 2 | 0.25 | 1 | 1 | ≤0.06 |
| PAM4179 | NDM-1 | B | 32 | 2 | 32 | 32 | 32 | 2 | 16 | >64 | 4 | >64 | >64 | >64 | 8 | >64 |
| PAM4917 | NDM-7 | B | 32 | 1 | 32 | 32 | 32 | 1 | 16 | >64 | 2 | >64 | >64 | >64 | 4 | >64 |
| PAM4795 | VIM-1 | B | 4 | ≤0.06 | 4 | 4 | 4 | ≤0.06 | 4 | >64 | 0.25 | >64 | >64 | 64 | 0.5 | 64 |
| PAM4798 | VIM-2 | B | 8 | ≤0.06 | 8 | 8 | 4 | ≤0.06 | 8 | 8 | 0.125 | 8 | 8 | 8 | 0.125 | 4 |
| PAM4887 | IMP-1 | B | 4 | 0.5 | 4 | 4 | 4 | 4 | 4 | 64 | 1 | 64 | 64 | 64 | 32 | 32 |
| PAM4888 | IMP-4 | B | 4 | 0.5 | 4 | 2 | 4 | 2 | 2 | 32 | 1 | 32 | 32 | 32 | 32 | 8 |
| PAM4196 | IMP-13 | B | 1 | 0.5 | 1 | 1 | 1 | 1 | ND | 16 | 1 | 16 | 16 | 16 | 16 | ND |
| PAM4198 | IMP-15 | B | 1 | 0.125 | 1 | 1 | 1 | 1 | ND | 16 | 0.5 | 16 | 16 | 16 | 16 | ND |
| PAM4890 | IMP-19 | B | 1 | 0.25 | 1 | 1 | 1 | 1 | 0.5 | 64 | 2 | 32 | 32 | 32 | 32 | 16 |
| PAM4889 | IMP-26 | B | 8 | 2 | 8 | 8 | 8 | 8 | 4 | 64 | 8 | 64 | 64 | 64 | 64 | 32 |
| PAM4883 | GIM-1 | B | 32 | 0.5 | 16 | 32 | 16 | 0.5 | ND | 8 | 0.25 | 8 | 8 | 8 | 0.125 | ND |
| PAM4885 | SPM-1 | B | 32 | 16 | 32 | 32 | 32 | 0.5 | 16 | >64 | 64 | >64 | >64 | >64 | 2 | 64 |
| PAM4879 | CcrA | B | 2 | ≤0.06 | 2 | 2 | 2 | 1 | ND | 16 | 0.125 | 16 | 16 | 16 | 8 | ND |
| PAM4880 | L1 | B | 32 | 16 | 32 | 32 | 32 | 32 | 16 | 4 | 1 | 4 | 4 | 4 | 4 | 1 |
a
MICs for antibiotics alone and MICs of antibiotics with of BLIs that less than 4-fold lower than that of antibiotics alone are in boldfaced values.