Table 1.
Up-regulated genes in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs) stimulated with protein I/II. Confluent RA FLSs were stimulated with protein I/II for 4 hours or left untreated as controls. Total RNA was isolated, reverse-transcribed into 32P-labeled cDNA, and hybridized to Atlas human cancer cDNA expression arrays. The values are expression ratios (protein I/II/ control).
| RA patient | ||||
| Protein product of gene | GenBank accession no. | 1 | 2 | 3 |
| Matrix metalloproteinase 3 | X05232 | 5.0 | 190.3 | 6.0 |
| IL-6 | X04602 | 14.6 | 65.0 | 4.0 |
| Leukemia inhibitory factor | X13967 | 10.8 | 15.9 | 5.0 |
| Interferon regulatory factor 1 | X14454 | 9.4 | 15.3 | 3.4 |
| Fibroblast growth factor-5 | M37825 | 3.5 | 2.9 | 5.7 |
| CDC27Hs protein | U00001 | 7.4 | - | 10.6 |
| Interferon gamma antagonist | A25270 | 5.4 | - | 13.0 |
| Integrin αE | L25851 | 3.9 | - | 3.5 |
| Vimentin | X56134 | 3.2 | - | 3.7 |
| Transmembrane protein SEX | X87852 | 3.8 | - | 3.0 |
| TRK-T3 | X85960 | - | - | 7.3 |
| Collagen I α2 subunit | X55525 | 5.7 | - | - |
| Transforming growth factor β2 | M19154 | - | - | 4.8 |
| Cell division protein kinase 4 | M14505 | 4.7 | - | - |
| Bone morphogenetic protein 2A | M22489 | - | 4.5 | - |
| Rho-related GTP-binding protein RhoE | X95282 | 4.3 | - | - |
| Cyclin H | U11791 | 4.3 | - | - |
| Tyrosine-protein kinase receptor UFO | M76125 | 3.9 | - | - |
| Tissue inhibitor of metalloproteinase 1 | X03124 | 3.9 | - | - |
| BIGH3 | M77349 | 3.8 | - | - |
| Ras-related C3 botulinum toxin substrate 1 | M29870 | 3.6 | - | - |
| Transforming protein RhoA H12 | L25080 | 3.5 | - | - |
| CD9 antigen | M38690 | 3.5 | - | - |
| Notch homolog | M99437 | 3.4 | - | - |
| Integrin β8 | M73780 | 3.4 | - | - |
| Secreted protein acidic and rich in cysteine | J03040 | 3.2 | - | - |
| α2-Macroglobulin receptor | X13916 | 3.1 | - | - |
| Fibronectin 1 | X02761 | 3.0 | - | - |
-, not found or <3.0.