Figure 1.

Clk^out mutants have impaired performance in multiple mobility associated assessments. Clk^out mutant males showed reduced endurance even at five days of age compared to background controls (A). Each data point depicts time to exhaustion for 80% of flies in an individual vial (n = 8 vials of 20 flies each) analyzed via log rank. Clk^out mutants also have reduced climbing speed across the first three weeks of life (B). Each data point represents the average climbing speed over four pictures taken daily (n = 5 vials of 20 flies each) analyzed longitudinally via a two-way ANOVA. The trend line represents the slope of linear regression. The flight performance of Clk^out mutants is also reduced (C). Average landing height between the two groups (n = 8 vials of 20 flies each) was compared using a t-test. Clk^out mutants showed no improvement in endurance following three weeks of chronic exercise training while the control w¹¹¹⁸ flies showed a significant improvement, n = 8 vials of 20 flies each (D). * p < 0.05, ** p < 0.001, ns is not significant.