TABLE 2.
Primers used in this study
| Primer | DNA sequence from 5′ to 3′ (restriction site)a |
|---|---|
| For gene cloning | |
| spoT-01 | ATGCGAATTCGAGATTTTAACCTATCCTTGACAC (EcoRI) |
| spoT-02 | ATGCCTGCAGCCATGATGCCATTCTTGAAA (PstI) |
| Cj0571-01 | ATGCGAATTCATGCAAGAAAATTTCATACGC (EcoRI) |
| Cj0571-02 | ATGCCTGCAGTCCCGTTGTAGCATCTTTTG (PstI) |
| hrcA-01 | ACGTGGTACCAATAGAGTGCTAGATATGAAGGAA (KpnI) |
| hrcA-02 | ACGTGGTACCGATGGTTTTCACTATCTACATGAA (KpnI) |
| hspR-01 | ACGTGGTACCGGAAAACAAAGCAGAAATGGAGC (KpnI) |
| hspR-02 | ACGTGGTACCGCACCACTGCGGTTGAAGAAA (KpnI) |
| For inverse PCR | |
| spoT-03 | ATGCGGATCCAAAACAGGAAAAGCAAAAGCA (BamHI) |
| spoT-04 | ATGCGGATCCAATGCCTTTTCTTTGCAT (BamHI) |
| Cj0571-03 | ATGCGGATCCGCTTAATTTTCCCAAAGCAAA (BamHI) |
| Cj0571-04 | ATGCGGATCCAGAACTGAAAATACGGCTAGAAGA (BamHI) |
| hrcA-03 | ACGTCCATGGATTGCTTCTATAGCAAATCAA (NcoI) |
| hrcA-04 | ACGTGCGGCCGCAAGCTCTTAGATTGTCAAATTCAC (NotI) |
| hspR-03 | ACGTCCATGGTTGCCATCGGTTCTACTTGG (NcoI) |
| hspR-04 | ACGTGCGGCCGCGAATCAATCTTGCTGGAGTA (NotI) |
a
The restriction sites used for cloning are highlighted in bold and indicated in parentheses.