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. 2005 Mar;73(3):1811–1819. doi: 10.1128/IAI.73.3.1811-1819.2005

FIG. 2.

FIG. 2.

S. aureus Newman, S. aureus RN6390, S. epidermidis 1457, and their ica mutants were grown anaerobically in chamber slides for 48 h. In panels A through F, culture broth was stained by indirect immunofluorescence to detect PIA. PIA was not detected in S. aureus strain Newman (panel A). In contrast, PIA was strongly marked by immunofluorescence in strain S. aureus RN6390 (panel C) and S. epidermidis 1457 (panel E) under anaerobic in vitro growth. All three ica mutants (panels B, D, and F) were PIA negative. WT, wild type. Original magnification, ×1,000.