TABLE 1.
Oligonucleotide primers and LightCycler hybridization probes
| Target gene | GenBank accession no. | Primer | Sequencea |
|---|---|---|---|
| S. aureus icaA | AF086783 | icaU | TGGCTGTATTAAGCGAAGTC |
| icaL | TTCCAAAGACCTCCCAATGT | ||
| icaFL | TGTCGACGTTGGCTACTGGGAT-FL | ||
| icaLC | Red640-CTGATATGATTACCGAAGATATTGCAGT-ph | ||
| S. aureus gyr | D10489 | gyrU | TTATGGTGCTGGGCAAATACA |
| gyrL | CACCATGTAAACCACCAGATA | ||
| gyrFL1 | ATTTTAACTGTTTTACATGCTGGTGGTAA-FL | ||
| gyrLC1 | Red640-TTTGGCGGTGGCGGATACA-ph | ||
| S. epidermidis icaA | U43366 | Epi-icaU | CAATCTCTTGCAGGAGC |
| Epi-icaL | CTTGAGCCCATCGAACC | ||
| Epi-icaFL1 | CAAAGATGTAGGTTATTGGGATACTGACA-FL | ||
| Epi-icaLC1 | Red640-ATTACTGAGGATATTGCTGTTTCATGGA-ph | ||
| S. epidermidis gyr | AF314404 | Epi-gyrU | GGTATGTATATTGGTTCAACTTCAG |
| Epi-gyrL | GTGAAGACCGCCAGAT | ||
| Epi-gyrFL2 | GGGTTGCACCATTTAGTATGGGAAA-FL | ||
| Epi-gyrLC2 | Red640-TGTTGATAATAGTATTGACGAGGCATTAGC-ph |
a
FL, fluorescein; Red640, LightCycler Red640-N-succinimide ester; ph, 3′-phosphate.