TABLE 1.

Real-time quantitative reverse transcriptase PCR probes and primers

RNA target	Probe sequence (5′-3′)	Forward primer sequence (5′-3′)	Reverse primer sequence (5′-3′)
18 SrRNA	ACCGGCGCAAGACGCACCAG	CGCCGCTAGAGGTGAAATTCT	CATTCTTGGCAAATGCTTTCG
TLR1	TCAGCCTCAAGCATTTGGACCTCTCCT	TGATCTTGTGCCACCCAACA	GCAGGGCATCAAAGGCATTA
TLR2	CGTTTTTACCACCCGGATCCCTGTACT	AAGATGCGCTTCCTGAATTTG	TCCAGCGTCTGAGGAATGC
TLR4	CCTGGTGTAGCCATTGCTGCCAACA	CCTCTGCCTTCACTACAGAGACTTT	GTGGAAGCCTTCCTGGATGA
TLR5	TGGTAATATCTCCCTGTTCTTCAGACGGCA	AACTTGACTTGCTCATAGGTGTGATC	CAGCCTCGGAAAAAGGCTATC
TLR6	AGCCAAGACAGAAAACCCATCGTGGG	TCTGGGATAGCCTCTGCAACA	GGCGCAAACAAAGTGGAAAC
TLR9	CGACCATGCCCCCAATCCCTG	TGATGTGGGTGGGAATTGC	GGGACTTTTGGCCACATTCTAT
MD2	TCTTTTCGACGCTGCTTTCTCCCATATTG	GGAGATATTAAATCATGTTGCCATTTATT	ACCACTGTTGCTTCTCAGATTCAG
MyD88	CCCTTGGTCGCGCTTAACGTGG	CTGGACTCCTTCATGTTCTCCAT	GATAGGCGGCGCCTCACT
TIRAP	TGCCGTGTGCTGCTCATCACTCC	TGCCAGGCACTGAGTCGTAGT	GGCCTGCAGCATCTGGTACT
TNF-α	TCAGCCTCTTCTCATTCCTGCTTGTGG	TCCAGGCGGTGCCTATGT	CGATCACCCCGAAGTTCAGT
iNOS	CTTCCGGGCAGCCTGTGAGACCT	CGCAGCTGGGCTGTACAA	TGATGTTTGCTTCGGACATCA

^aPrimers and probes were designed by using the Primer Express software program (PE Applied Biosystems). The probes were labeled with the fluorescent reporter dye 5-carboxyfluorescein at the 5′ end and with the quencher N,N,N,N′-tetramethyl-6- carboxyrhodamine at the 3′ end.