Figure 5.

VER-82576 could inhibit TGEV infection. (A) Schematic diagram of ST cells treated with the HSP90 inhibitors VER-82576 and KW-2478. Blue double-headed arrows indicate KW-2478 or VER-82576 treatment, and red double-headed arrows indicate TGEV incubation. (B,F) The relative mRNA level of the TGEV M gene was determined by qRT-PCR. (C,G) Viral titer in the supernatant was analyzed by TCID₅₀ and (D,H) N protein level was determined by Western blot. (E,I) The band density of the protein was quantified using Image J software, and the TGEV-N to ACTB ratios were normalized to the control. * means p ≤ 0.05, ** means p ≤ 0.01, *** means p ≤ 0.001, ns means p > 0.05.