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. 2023 Nov 4;24(21):15971. doi: 10.3390/ijms242115971

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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VER-82576 had no influence on the entry stage of TGEV infection. (A) Schematic diagram of ST cells treated with VER-82576 before (−1–0 h) or during (0–2 h) TGEV incubation. Blue double-headed arrows indicate VER-82576 treatment, and red double-headed arrows indicate TGEV incubation. (B,F) The relative mRNA level of TGEV M gene was determined by qRT-PCR. (C,G) Viral titer in the supernatant was analyzed by TCID₅₀, and (D,H) N protein level was determined by Western blot. (E,I) The band density of the protein was quantified using Image J software, and the TGEV-N to ACTB ratios were normalized to the control.