VER-82576 inhibits TGEV infection at the early stage of viral replication. (A) Schematic diagram of ST cells treated with VER-82576 at different time points after viral entry. Blue arrows indicate the time point at which VER-82576 treatment began, and the blue line indicates the presence of VER-82576. Red down arrows and the red line indicate TGEV incubation. (B) TGEV M gene mRNA levels, (C) viral titers in the cell supernatants, and (D) N protein levels in VER-82576 treated ST cells. (E) The band density of the protein was quantified using Image J software, and the TGEV-N to ACTB ratios were normalized to the control. * means p ≤ 0.05, ** means p ≤ 0.01, ns means p > 0.05.