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Primer sequences used for qRT-PCR.
| Genes | Sequences (5′-3′) | Size (bp) |
|---|---|---|
| TGEV M | F: GTGGAGAACGCTATTGTGCTAT | 258 |
| R: AAATCGTAAGAGCCAAAACAAC | ||
| IL-6 | F: GATGCTTCCAATCTGGGTTC | 218 |
| R: ATTTGTGGTGGGGTTAGGG | ||
| IL12 | F: AACCACCTGGACCATCTCA | 211 |
| R: CCTCCACTGTGCTGGTTTT | ||
| TNF-α | F: TCCTCACTCACACCATCAGC | 222 |
| R: GCCCAGATTCAGCAAAGTCC | ||
| CXCL10 | F: AATCTACCTCTGCCATCATCTC | 373 |
| R: AGTAGAAGCCCACGGAGTAAAG | ||
| CXCL11 | F: AACTATTCAAGGCTTCCCCAT | 201 |
| R: ACATTTGCTTGCTTTGATTTG | ||
| ACTB | F: CTTCCTGGGCATGGAGTCC | 201 |
| R: GGCGCGATGATCTTGATCTTC |
