TABLE 1.
B. pertussis-infected MDDC are phenotypically maturea
| Stimulusb | Mean expression ± SE of the indicated surface marker (isotype-matched control antibody)
|
||
|---|---|---|---|
| CD80 (MFI) | CD83 (% positive cells) | HLA-DR (MFI) | |
| B. pertussis (n = 12) | 44.29 ± 7.74c (2.65 ± 0.22) | 48.43 ± 5.89c (0.05 ± 0.02) | 156.75 ± 26.75 (2.26 ± 0.27) |
| HI B. pertussis (n = 6) | 51.08 ± 11.32c (2.50 ± 0.30) | 43.67 ± 12.62c (0.10 ± 0.03) | 150.61 ± 40.46 (2.60 ± 0.27) |
| LPS (n = 18) | 38.09 ± 2.7c (2.48 ± 0.24) | 55.18 ± 4.35c (0.06 ± 0.01) | 179.91 ± 22.77 (2.30 ± 0.25) |
| None (n = 18) | 14.85 ± 1.64 (2.45 ± 0.26) | 3.78 ± 0.85 (0.06 ± 0.02) | 114.16 ± 29.19 (2.72 ± 0.24) |
a
iMDDC were treated with the indicated stimuli and analyzed for surface markers.
b
n, number of experiments.
c
P < 0.001 by Student's t test, in each column, for comparison between stimulated and unstimulated (none) DC cultures.