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Strain genotypes and reverse latex agglutination test for TDH production
| Test strain or purified toxin | Toxin genotypea | TDH titera
|
||
|---|---|---|---|---|
| Mannitol- peptone (18 h) | DMEM- FBS (18 h)b | DMEM- FBS (3 h)b | ||
| WA10290 | tdh+/trh+ | 2 | 2 | ND |
| BCC36 | tdh+/Δtrh | 128 | 32 | ND |
| NY477 | tdh+/Δtrh | 64 | ||
| Purified TDH (1 HU/ml) | NA | 1,024 | ||
| ATCC 17082 | Δtdh/trh+ | NA | ||
| BCC34 | Δtdh/Δtrh | NA | ||
| DAL1094 | Δtdh/Δtrh | 0 | ||
| BCE306 | Δtdh/Δtrh | NA | ||
| BCE515 | Δtdh/Δtrh | NA | ||
NA, not applicable; ND, not detected.
FBS, fetal bovine serum.
