TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or phenotype | Source or reference |
|---|---|---|
| Strains | ||
| E2348/69 | O127:H6 EPEC isolate | 14 |
| II104 | E2348/69 slyA | This laboratory |
| II150 | E2348/69 hosA | This study |
| II151 | E2348/69 slyA hosA | This study |
| II152 | E2348/69 fliC | This study |
| DH5α | F−endA hsdR17 (rK− mk+) supE44 thi-1 recA1 gyrA96 φ80 lacZ | 7 |
| MG1655 | E. coli K-12 | 3 |
| S17-1 λpir | λpir lysogen thi pro HsdR−hsdM+recA RP4 2-Tc::Mu-Km::Tn7 (Tpr Smr) | 26 |
| Plasmids | ||
| pBAD24 | Expression vector with pBAD promoter and araC negative regulator | 6 |
| pDM4 | pir-dependent suicide vector, sacB double-cross over selection; Cmr | 19 |
| pUC57/T | T-cloning vector; Apr | Clontech |
| pII19 | pUC57/T containing slyA | This laboratory |
| pII30 | ptrc99A containing slyA, inducible by IPTG | This laboratory |
| pMJFA10 | pDM4 suicide plasmid containing hosA deletion (from nucleotide 4 to 375) | This study |
| pMJFA12 | pBAD24 containing hosA, inducible by arabinose | This study |
| pMJFA18 | PCR-amplified hosA promoter and gfp cloned into pSB377; Ampr | This study |
| pKB100 | PCR-amplified fliC promoter cloned into pSB377; Ampr | This study |
| pMJFA21 | PCR-amplified hosA cloned into pPROEX; Ampr | This study |
| pMJFA22 | PCR-amplified LEE1 operon promoter fused to luxCDABE in pSB377; Apr | This study |
| pHW100 | PCR-amplified LEE4 operon promoter fused to luxCDABE in pSB377; Apr | This study |
| pPROEX HT | IPTG-inducible expression vector, trc promoter, His, lacIq, f1 intergenic region; Apr | Invitrogen |
| pSB377 | luxCDABE reporter operon, rop, ColE1 ori; Apr | 33 |
| ptrc99A | ITPG-inducible expression vector, trc promoter; lacIqrrnBt1rrnBt2; Apr | Pharmacia |