Strains |
|
|
E2348/69 |
O127:H6 EPEC isolate |
14 |
II104 |
E2348/69 slyA
|
This laboratory |
II150 |
E2348/69 hosA
|
This study |
II151 |
E2348/69 slyA hosA
|
This study |
II152 |
E2348/69 fliC
|
This study |
DH5α |
F−endA hsdR17 (rK− mk+) supE44 thi-1 recA1 gyrA96 φ80 lacZ
|
7 |
MG1655 |
E. coli K-12 |
3 |
S17-1 λpir
|
λpir lysogen thi pro HsdR−hsdM+recA RP4 2-Tc::Mu-Km::Tn7 (Tpr Smr) |
26 |
Plasmids |
|
|
pBAD24 |
Expression vector with pBAD promoter and araC negative regulator |
6 |
pDM4 |
pir-dependent suicide vector, sacB double-cross over selection; Cmr
|
19 |
pUC57/T |
T-cloning vector; Apr
|
Clontech |
pII19 |
pUC57/T containing slyA
|
This laboratory |
pII30 |
ptrc99A containing slyA, inducible by IPTG |
This laboratory |
pMJFA10 |
pDM4 suicide plasmid containing hosA deletion (from nucleotide 4 to 375) |
This study |
pMJFA12 |
pBAD24 containing hosA, inducible by arabinose |
This study |
pMJFA18 |
PCR-amplified hosA promoter and gfp cloned into pSB377; Ampr
|
This study |
pKB100 |
PCR-amplified fliC promoter cloned into pSB377; Ampr
|
This study |
pMJFA21 |
PCR-amplified hosA cloned into pPROEX; Ampr
|
This study |
pMJFA22 |
PCR-amplified LEE1 operon promoter fused to luxCDABE in pSB377; Apr
|
This study |
pHW100 |
PCR-amplified LEE4 operon promoter fused to luxCDABE in pSB377; Apr
|
This study |
pPROEX HT |
IPTG-inducible expression vector, trc promoter, His, lacIq, f1 intergenic region; Apr
|
Invitrogen |
pSB377 |
luxCDABE reporter operon, rop, ColE1 ori; Apr
|
33 |
ptrc99A |
ITPG-inducible expression vector, trc promoter; lacIqrrnBt1rrnBt2; Apr
|
Pharmacia |