TABLE 2.

Oligonucleotides used in this study

Oligonucleotide	Oligonucleotide sequence (5′→3′)a	Use
VvpACl-1A	CTTTCTGGTTGGTCGACCATCACCGC	pilA mutant construction
VvpACl-2	CTGTTGCTGATGCCCGGGCAACTTC	pilA mutant construction
VvpACl-3A	AAACTGAAGTTGCCCGGGCATCAGC	pilA mutant construction
VvpACl-4	CGAGCTGTCTAGAAGTCGCAACAC	pilA mutant construction
VvpA-N-pFLAG	CCAAGAAACAACAACAAGACAAAGTCACCTTGATTG	Recombinant PilA
VvpA-C-pFLAG	TTGGCCTGACGCAGAATTCTGGAGAGATTC	Recombinant PilA
VvpAF3	GGTGTGATTTCCACTCATC	pilA probe
VvpBR6	CTCTCGACACGCTTGTTC	pilA probe
VvpilDRT	TACCTGACGAGCGACAATG	RT of pilD
VvpAF32	TGGCTGCTGTTGCTATTC	PCR of pilA
VvpAR2	GGTCCACCACTAGTACCAAC	PCR of pilA
VvpBF11	CTG GTT TAA AAG AGA TTC AC	PCR of pilB
VvpBR7	TGC TCG AGC TGT CGA GAA GTC	PCR of pilB
VvpilCF2	CCAGTGTGTCAGTTTTCACGAGG	PCR of pilC
VvpilCR2	TCGCCGCTTTAGATAAGACCCC	PCR of pilC
VvpilDF1	TGAATACAACATCACACCACCGAC	PCR of pilD
VvpilDR1	CCTTCTTTCCCTGTCAGCAATCTG	PCR of pilD

^aUnderlined nucleotides represent engineered restriction enzyme sites.