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Phosphate uptake rates of the pstS1 and pstS2 knockout (k/o) mutant and the wild-type M. tuberculosis (M. tub. wt.) strains. The uptake rate of orthophosphate (10 mCi/mmol; Amersham-Pharmacia) (expressed in nanomoles of Pi per milligram of mycobacterial protein extract) of the parental wild type and the pstS1 and pstS2 mutant derivatives of M. tuberculosis H37Rv were measured at 0.5 (A), 2 (B), 5 (C), 10 (D), and 25 (E) μM Pi.
