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. 2005 Mar;73(3):1532–1542. doi: 10.1128/IAI.73.3.1532-1542.2005

FIG. 6.

FIG. 6.

Test for protection by anti-LcrV antibody in the absence of effector Yops. C57BL/6 mice were treated with anti-YopM (circles) or anti-LcrV (squares) antibody and infected with Y. pestis strain KIM5-MYM, which is unable to express any of the six effector Yops. At various times postinfection, viable numbers (CFU) were determined in spleens (A) and livers (B) of 3 mice per treatment. The plates contained less than 30 colonies at 72 h for spleen and after 48 h for liver (denoted by arrows). In a modified protocol to eliminate possible limitation by the amount of anti-LcrV antibody given the mice, the preimmunizing dose of anti-LcrV and anti-YopM antibody was 500 μg; on days 1 (6 hpi) and 2 (30 hpi), the mice received 200-μg doses of antibody. CFU are shown for spleens (C) and livers (D). The doses given the mice are plotted at time zero (triangles). Panels A and B represent one of two similar experiments. The modified protocol of panels C and D was done once. Error bars are ±1 standard deviation.