Table 2.
PCR mixture using various polymerase enzymes.
| Enzyme | Trade Name | Source | Volume (µL) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 10× Taq Buffer | 10 mM dNTP | Hot Start Taq | F-Primer | R-Primer | DNA Template | Water | |||
| GMbiolab | GenHot Taq DNA Polymerase | Gmbiolab Co., Ltd., Taipei, Taiwan | 2.5 | 0.5 | 0.50 | 0.5 | 0.5 | 2.0 | 18.50 |
| ZymesetBiotek | Hot start Taq DNA polymerase | ZymesetBiotek, Taipei, Taiwan | 2.5 | 0.5 | 0.25 | 0.5 | 0.5 | 2.0 | 18.75 |
| BioTools | TOOLS Hotstart Polymerase | Biotools, New Taipei City, Taiwan | 2.5 | 0.5 | 0.50 | 0.5 | 0.5 | 2.0 | 18.50 |
| BioHelix | nanoTaq Hot-Start DNA Polymerase | Bio-Helix Co., Ltd., New Taipei City, Taiwan | 2.5 | 0.5 | 1.25 | 0.5 | 0.5 | 2.0 | 17.75 |
| ThermoFisher | Invitrogen™ Platinum™ Taq DNA Polymerase 1 | ThermoFisher, Waltham, MA, USA | 2.5 | 0.5 | 0.10 | 0.5 | 0.5 | 2.0 | 18.15 |
| BiOptic | DirectGOTM PreMix-CE 2 | BiOptic Inc., New Taipei City, Taiwan | Premix: 12.5 | 0.5 | 0.5 | 2.0 | 9.50 | ||
1 Note: 0.75 µL of Mg2+ was added to the mixture as this chemical was not included in the buffer. 2 PCR reagent from Bioptic was provided in a ready-to-use master mix containing reaction buffer, dNTPs, glycerol, PCR enhancers, and Hot Start Taq DNA polymerase as a 2-fold concentration.