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. 2005 Mar;73(3):1568–1577. doi: 10.1128/IAI.73.3.1568-1577.2005

FIG. 4.

FIG. 4.

FIG. 4.

FIG. 4.

CpG induces maturation of UV-EB-pulsed DC. (A) Effect of CpG on the surface expression of CD40 and CD86. DC were left untreated or incubated with either UV-EB, UV-EB and CpG, or CpG alone. After 48 h, DC were stained for CD11c and either CD40 or CD86 and analyzed by FACS. Results shown are the percentage of CD11c+ cells expressing either CD40 or CD86. Results represent the mean ± SD from five independent experiments. (B) Allogeneic T-cell proliferation induced by DC upon exposure to UV-EB and CpG. Purified DC were left untreated or incubated with CpG (30 μg/ml), UV-EB alone, or UV-EB and CpG (30 μg/ml) and incubated for 48 h. Tritiated thymidine was added for an additional 24 h before cells were harvested and analyzed for thymidine incorporation. Experiments were performed on three separate occasions, with similar results. Results represent the mean ± SD from one experiment and show the number of cpm calculated per 100 DC. (C) Cytokine production by DC upon exposure to UV-EB and CpG. DC were left untreated or incubated with CpG (30 μg/ml), UV-EB, or UV-EB and CpG (30 μg/ml) for 48 h before the supernatants were analyzed for cytokine production by ELISA. Experiments were performed on three separate occasions, with similar results. Results represent the mean ± SD from one representative experiment. *, P < 0.01; **, P < 0.001 (both comparing UV-EB-pulsed DC and DC exposed to UV-EB and CpG).