TABLE 2.
Sequences of oligonucleotide primers used for PCR amplification and cloning
| Gene | Primera | Sequence (5′-3′)b |
|---|---|---|
| psaA | 21(F) | AATCGTCATATGGCCATGGGCgctagcggaaaaaaagatgcagcttc |
| 308(R) | ATTCCCCTCGAGAAGCTTGGATCCtgccaatccttcagcaatctt | |
| ppmA | 22(F) | AATCGTCATATGGCCATGGGCtcgaaagggtcagaaggtgcagac |
| 313(R) | ATTCCCCTCGAGAAGCTTGGATCCttcgtttgatgtactactgcttgagc | |
| pspA | 26(F) | AATCGTCATATGGCCATGGGCcctacttttgtaagagcagaagaag/tc |
| 409(R) | ATTCCCCTCGAGAAGCTTGGATCCaccgttttcttgtttccagcc |
a
Numbers represent the first amino acid encoded by the forward (F) primers or the last amino acid encoded by the reverse (R) primers, respectively. All numbering is based on pre-pro amino acid sequences for each gene.
b
Restriction sites in each primer relevant to this study (NcoI for “F” primers and XhoI for “R” primers) are in boldface; nucleotides in lowercase are derived from sequences of each gene deposited in GenBank.