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Fur-dependent repression of PbhuR. (A) pDJM41 encodes a 199-bp segment of DNA encompassing the rhuR-bhuR intergenic region containing PbhuR (thick line) which is fused to lacZYA (shaded box) (not drawn to scale). (B) Both wt 4169rif and 4169riffur were transformed with pDJM41. Strains were cultured in BHI broth supplemented with 36 μM FeSO4 (High Fe) or 100 μM EDDHA (Low Fe). β-Galactosidase activities were measured from log-phase cultures.
