TABLE 1.
Time (h) | % of cells
|
|||||
---|---|---|---|---|---|---|
CD3
|
CD19
|
CD14
|
||||
Control | 1,000 ng of toxin A/ml | Control | 1,000 ng of toxin A/ml | Control | 1,000 ng of toxin A/ml | |
0 | 58.9 ± 0.8 | 58.9 ± 0.7 | 12.3 ± 1.5 | 12.1 ± 1.5 | 8.9 ± 1.8 | 8.4 ± 1.3 |
2 | 59.5 ± 1.9 | 60.4 ± 2.9 | 10.9 ± 4.1 | 11.1 ± 4.1 | 8.3 ± 1.2 | 4.6 ± 1.8 |
5 | 57.1 ± 3.9 | 62.3 ± 3.4 | 9.8 ± 2.7 | 11.4 ± 3.9 | 9.3 ± 1.7 | 0.4 ± 0.3 |
24 | 59.3 ± 4.0 | 58.4 ± 6.9 | 12.6 ± 2.8 | 10.6 ± 0.6 | 9.2 ± 2.4 | 0.2 ± 0.1 |
Data for events specific for T cells (CD3), B cells (CD19), and monocytes (CD14) in control and toxin A-exposed PBMNCs are shown. Following isolation, PBMNCs were exposed to 1,000 ng of purified C. difficile toxin A per ml for 2 to 24 h before analysis by flow cytometry. The data are means ± standard errors of the means for cells from three experiments.