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. 2005 Mar;73(3):1625–1634. doi: 10.1128/IAI.73.3.1625-1634.2005

TABLE 1.

Flow cytometric analysis of PBMNCs exposed to C. difficile toxin Aa

Time (h) % of cells
CD3
CD19
CD14
Control 1,000 ng of toxin A/ml Control 1,000 ng of toxin A/ml Control 1,000 ng of toxin A/ml
0 58.9 ± 0.8 58.9 ± 0.7 12.3 ± 1.5 12.1 ± 1.5 8.9 ± 1.8 8.4 ± 1.3
2 59.5 ± 1.9 60.4 ± 2.9 10.9 ± 4.1 11.1 ± 4.1 8.3 ± 1.2 4.6 ± 1.8
5 57.1 ± 3.9 62.3 ± 3.4 9.8 ± 2.7 11.4 ± 3.9 9.3 ± 1.7 0.4 ± 0.3
24 59.3 ± 4.0 58.4 ± 6.9 12.6 ± 2.8 10.6 ± 0.6 9.2 ± 2.4 0.2 ± 0.1
a

Data for events specific for T cells (CD3), B cells (CD19), and monocytes (CD14) in control and toxin A-exposed PBMNCs are shown. Following isolation, PBMNCs were exposed to 1,000 ng of purified C. difficile toxin A per ml for 2 to 24 h before analysis by flow cytometry. The data are means ± standard errors of the means for cells from three experiments.