TABLE 2.

Incubation with toxin A decreases the numbers of CD14-positive events in a time- and dose-dependent manner^a

Concn of toxin A (ng/ml)	No. of CD14-positive events (mean ± SEM)
	2 h	5 h	24 h
0	8,308 ± 1,218	9,354 ± 1,795	9,192 ± 2,390
1	8,420 ± 1,293	7,860 ± 1,320	7,850 ± 2,393
10	8,400 ± 1,213	9,223 ± 2,298	4,430 ± 547
100	8,297 ± 1,138	7,460 ± 2,300	260 ± 80b
1,000	4,638 ± 1,854b	400 ± 340c	230 ± 152d

^aPBMNCs were labeled with anti-CD14-PE antibodies after incubation with 10-fold concentrations of toxin for 2, 5, and 24 h. Cells were then analyzed by single-color flow cytometry, and CD14-positive events were enumerated (n = 3).

^bP < 0.05.

^cP < 0.001.

^dP < 0.01.