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. 2005 Mar;73(3):1377–1385. doi: 10.1128/IAI.73.3.1377-1385.2005

FIG. 6.

FIG. 6.

FimYZ activation of the hilE promoter requires the presence of sequences in the hilE promoter in E. coli and S. enterica serovar Typhimurium. Expression values for the hilE-lacZY reporter plasmids in E. coli are shown in panel A. The E. coli strains DH12S/pMAB69, DH12S/pMAB69/pISF182, DH12S/pMAB102, and DH12S/pMAB102/pISF182 were grown with shaking overnight in Lennox broth. Expression values for hilE-lacZY reporter plasmids in S. enterica serovar Typhimurium are shown in panel B. The S. enterica serovar Typhimurium strains SL1344 ΔhilE/pMAB69, SL1344 ΔhilE/pMAB69/pISF182, SL1344 ΔhilE/pMAB102, and SL1344 ΔhilE/pMAB102/pISF182 were grown with shaking overnight in Lennox broth. Plasmid pMAB69 encodes the hilE-lacZY reporter. Plasmd pMAB102 encodes the hilE-lacZY reporter with putative FimYZ binding sites deleted from the promoter. Plasmid pISF182 encodes functional fimY and fimZ genes. β-Galactosidase assays were performed at least three times.