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. 2005 Mar;73(3):1377–1385. doi: 10.1128/IAI.73.3.1377-1385.2005

FIG. 7.

FIG. 7.

Mapping the hilE transcriptional start site in relation to the putative translation start site for hilE and the putative FimYZ binding site. The primer extension products from pMAB69 and pMAB102 were primed with hilE23 and run alongside dideoxy sequencing reactions (lanes marked CTAG). The 5′ end of the hilE mRNA appears to initiate at two positions. The first mRNA initiates at an adenosine 55 bp upstream of the putative ATG start codon. The second transcription start site initiates at an adenosine 148 bp upstream of the putative ATG start codon. This second transcript starts 54 bp downstream of the putative FimYZ binding sites.