TABLE 1.

Bacterial strains and plasmids used in the study

Strain or plasmid	Descriptiona	Reference(s) or source
Escherichia coli DH12S	mcrA Δ(mrr-hsdRMS-mcrBC) F′ lacIq ΔM15	Invitrogen
Salmonella enterica serovar Typhimurium
BJ2710	SL1344 derivative containing LT2 fimH gene	This work
BJ2710 fimZ-kan	fimZ::kan in BJ2710, Kanr	This work
BJ2462	hilE::cam in SL1344, Camr	6
LB5010	Strain LT2 containing a complete fim gene cluster	56
LBZ100	LB5010 fimZ::kan, Kanr	56
SL1344	Wild-type virulent strain	54
SL1344 fimZ-kan	fimZ::kan in SL1344 transduced from LBZ100, Kanr	This work
SL1344H3	SL1344 strain containing a fimH::kan insertion, Kanr	8
Plasmids
pACYC184	Camr Tetr	10
pISF182	fimYZ cloned into pACYC184, Camr	55
pLS31	pRW50 vector encoding −497 to +420 of hilA fused to lacZY, Tetr	43
pKD3	pANTSγ vector containing the chloramphenicol template gene cloned from pSC140, Ampr	17
pKD46	Temperature-sensitive red helper plasmid expressing araC-ParaB and γβexo from λ phage, Ampr	17
pMAB54	pGEM-T vector encoding hilE promoter from −886 to +121, Ampr	This work
pMAB55	pGEM-T vector encoding hilE promoter from −886 to +121 and promoterless lacZY genes downstream of the promoter, Ampr	This work
pMAB56	pZC320 vector encoding hilE promoter from −886 to +121, Ampr	This work
pMAB69	hilE promoter −886 to +121 cloned in pRW50, Tetr	This work
pMAB95	pGEM-T encoding the hilE promoter fragment −191 to +121, Ampr	This work
pMAB97	pGEM-T vector encoding the hilE promoter fragment −886 to −271, Ampr	This work
pMAB98	pGEM-T vector encoding the hilE promoter from −886 to +121 minus the −271 to −191 region, Ampr	This work
pMAB99	pMAB98 ligated into pRW50, Ampr Tetr	This work
pMAB102	pRW50 lacZY-expressing vector with the hilE promoter missing −271 to −191 relative to the putative translation start site inserted upstream to lacZY, Tetr	This work
pMRP9-1	GFP-expressing plasmid, Camr	E. P. Greenberg
pRTP::Tn5	Tn5 transposon inserted onto plasmid pRTP1 which carries the rpsL gene (Strs) Ampr Kanr	27, 46
pRW50	lacZ reporter vector, Tetr	31
pZC320	Mini-F, Ampr	45

^aTet^r, tetracycline resistant; Amp^r, ampicillin resistant; Kan^r, kanamycin resistant; Cam^r, chloramphenicol resistant.