FIG. 1.
Time course of disappearance of Bim, Puma, and Bad during chlamydial infection. At the indicated time points postinfection with C. trachomatis Triton X-100 extracts of Hep2 cell samples were prepared and analyzed by Western blotting against the Bcl-2-family proteins Bim, Puma, Bad, Bcl-2, Bcl-xL, Bax, and Bak. Detection of tubulin served as a loading control. The top and bottom panels were obtained by reprobing two membranes that contained samples from the same experiments. Degradation of Bim, Puma, and Bad was observed in at least three experiments.