FIG. 5.

Chlamydial infection leads to the degradation of activated (truncated) Bid. Jurkat or HeLa-fas cells were infected with C. trachomatis or left uninfected. After 24 h of infection cells were treated with MAb to the fas death receptor. In some experiments, the proteasome inhibitor MG132 (50 μM) was added at the same time, as indicated. After 6 h cells were harvested, and extracts were subjected to Western blotting and probed with an antibody that recognizes both Bid and tBid (although sensitivity for tBid is much lower than for Bid). Both Jurkat cells (left) and HeLa-fas cells (right) are shown. Chlamydial Hsp60 served as a control for chlamydial infection; detection of tubulin served as a loading control.