Table 2.
Chromatography and mass spectrometry parameters. This table represents the instrumentation settings used in the analysis method and the parameters applied. Settings are selected for the most favourable separation and ionisation of eicosanoids and fatty acids.
| Chromatography Setting | Parameter |
|---|---|
| Mobile phase composition | A = 2% IPA, 5mM Ammonium Acetate B = 100% IPA, 5mM Ammonium Acetate |
| Mobile phase flow rate (mL/min) | 0.21 |
| C18 column temperature (°C) | 40 |
| Source setting | Parameter |
| Gas temperature (°C) | 280 |
| Gas flow (L/min) | 14 |
| Nebulizer (psi) | 20 |
| Sheath gas temperature (°C) | 250 |
| Sheath gas flow (L/min) | 11 |
| Capillary: positive and negative polarity (V) | 3000 |
| Nozzle:positive and negative polarity (V) | 1500 |
| iFunnel setting | Parameter |
| High Pressure RF: positive polarity (V) | 150 |
| Low pressure RF: positive polarity (V) | 60 |