TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Characteristics | Reference or source |
|---|---|---|
| E. coli strains | ||
| DH5α | endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF) U169 deoR [Φ80dlacΔ(lacZ)M15] | 23 |
| SM10λpir | pirR6K | 30 |
| MM294/pRK2013 | Kanr; helper strain for matings | 4 |
| S. flexneri strains | ||
| SA100 | S. flexneri wild-type serotype 2a | 19 |
| SM100 | SA100 Strr | S. Seliger |
| SM169 | SM100 pstS::cam | 22 |
| UR005 | SM169 phoB::tet | This study |
| Plasmids | ||
| pHM5 | Allelic exchange vector | 21 |
| pMTLtet | Tetr gene from pBR322 in pMTL24 | S. Reeves |
| pLR29 | pGTXN3 with RP4 mobilization region from pGP704 | 22 |
| pLL1 | pWKS30 carrying phoBR | This study |
| pLL1::tet | pWKS30 carrying phoB::tet | This study |
| pLL2 | pHM5 carrying phoB::tet | This study |
| pLR83 | pLR29 carrying the phoA promoter | 22 |
| pPst1 | pWKS30 carrying pstSCABphoU | This study |
| pAB1 | pPst carrying R220Q mutation in pstA | This study |
| pWKS30 | Low-copy-number cloning vector | 32 |