TABLE 1.
Antigen-specific proliferation of spleen cells from genetically immunized mice
| Stimulationa | Stimulation indexb
|
||
|---|---|---|---|
| Naïve | Blank vector | synVW2-1 | |
| RPMIc | 1 ± 0.09 | 1 ± 0.37 | 1 ± 0.06 |
| RPMI + recVW2-1 | 1.49 ± 0.15 | 0.99 ± 0.01 | 3.55 ± 0.61d |
| RPMI + ConA | 22.89 ± 1.19 | 23.79 ± 0.18 | 21.62 ± 1.85 |
Proliferative responses of spleen cells were determined by incorporation of [3H]thymidine, after 72 h of culture in the presence of recVW2-1 (1 and 5 μg/ml) or concanavalin A (ConA) (1 μg/ml) as a control.
Mean ± standard deviation, calculated from the means of triplicate samples for radioactivity incorporation after a 18-h pulse with [3H]thymidine.
The absolute values for RPMI-stimulated cells were 16.87 ± 1.43, 15.7 ± 5.76, and 16.93 ± 1.03 kcpm (means ± standard deviations) for the naïve, blank vector, and synVW2-1 groups, respectively.
Absolute values for each experimental group were statistically different from the absolute values for RPMI-stimulated cells (P < 0.05).