TABLE 2.
Cytokine profiles of spleen cells from genetically immunized mice
| Spleen cellsa | Production (pg/ml) ofb:
|
|
|---|---|---|
| Interleukin-4c | IFN-γc | |
| Naïve | ≤7.8 | ≤31 |
| Blank vector | ≤7.8 | ≤31 |
| syn VW2-1 | ≤7.8 | 705.66 ± 32.62d |
a
Spleen cells from different groups were cultured in vitro in the presence of recVW2-1 (5 μg/ml) to quantify interleukin-4 and IFN-γ in the culture supernatants.
b
The values are means±standard deviations for triplicate representatives of at least two experiments.
c
The lower detection limits for interleukin-4 and IFN-γ were 7.8 and 31 pg/ml, respectively.
d
Statistically significant difference between naïve cells and immune cells (P < 0.01).