Figure 5.

Sarcomere α-tubulin networks are similar in wildtype (WT) and hypophosphatasia (HPP) muscles. (Top) TA muscles were treated with Mitospy Orange to visualize the mitochondrial network organization. In both WT and HPP muscles, the mitochondrial network took on similar linear organization. Scale bar: 2 µm (Center) The TA muscle α-tubulin networks were visualized by antibody staining. The microtubules of the WT and HPP muscles appear similar to one another, with both displaying rectilinear organization. (Bottom) The basic contractile subunit of muscles is the sarcomere, consisting of actin and myosin filaments between the z-line. Using SHG imaging, the sarcomere structures appeared to be similar between the WT and HPP groups. To determine if there were more sarcomeres in the WT than the HPP muscle, we determined the distance between the myosin bands and found no significant difference in the myosin band spacing between the groups (p = 0.105, n = 4/group). Independent t-tests assessed mean differences between WT and HPP genotypes. Scale bar: 10 µm.