FIG. 5.

Multimodal imaging for the establishment of imaging-guided experimental treatment strategies. Coregistration of [¹⁸F]FIAU-, [¹¹C]MET-, [¹⁸F]FDG-PET and MRI before (left column) and after (right column) targeted application (stereotactic infusion) of a gene therapy vector. The region of specific [¹²⁴I]-FIAU retention (68 h) within the tumor after LIPO-HSV-1-tk transduction (white arrow) resembles the proposed tissue dose of vector-mediated gene expression and shows signs of necrosis (cross right column; reduced methionine uptake [MET] and glucose metabolism [FDG]) after ganciclovir treatment. Reproduced with permission from Jacobs et al. Positron-emission tomography of vector-mediated gene expression in gene therapy for gliomas. Lancet 358:727–729. Copyright © 2001, Elsevier Limited. All rights reserved.¹²⁰