TABLE 3.
Effect of K. ascorbata SUD165 on the toxicity of nickel to canola and tomato seedlings grown in gnotobiotic growth pouches or in soil in pots
| Expta | Organ | Ni2+ concn (mM) | TIb | ||
|---|---|---|---|---|---|
| Ni2+ only | Ni2+ + cellsc | Ni2+ + cellsd | |||
| 1 | Shoots | 1 | 0.48 | 0.75 | NDe | 
| Roots | 1 | 0.57 | 0.85 | ND | |
| 2 | Shoots | 2 | 0.22 | 0.49 | 0.71 | 
| Roots | 2 | 0.11 | 0.34 | 0.54 | |
| 3 | Shoots | 2 | 0.22 | 0.47 | ND | 
| Roots | 2 | 0.08 | 0.32 | ND | |
| 4 | Shoots | 1 | 0.44 | 0.71 | 0.81 | 
| Roots | 1 | 0.49 | 0.73 | 0.87 | |
| 5 | Shoots | 6 | 0.12 | ND | 0.18 | 
| Roots | 6 | 0.23 | ND | 0.33 | |
| 6 | Shoots | 6 | 0.18 | ND | 0.41 | 
| Roots | 6 | 0.23 | ND | 0.36 | |
| 7 | Shoots | 3 | 0.54 | ND | 0.70 | 
| Roots | 3 | 0.57 | ND | 0.80 | |
| 8 | Shoots | 4 | 0.42 | ND | 0.59 | 
| Roots | 4 | 0.35 | ND | 0.56 | |
Experiments were performed with canola (experiments 1 to 6) and tomato (experiments 7 and 8) seedlings grown in gnotobiotic growth pouches (experiments 1 to 4) or in soil in pots (experiments 5 to 8).
The values for the different treatments differ significantly from the untreated control and from each other (P < 0.001). Each value (including the seeds grown in the absence of nickel, i.e., TI = 1.0) reflects the mean of the response of 50 to 60 seeds.
K. ascorbata SUD165 cells diluted to an absorbance at 600 nm of 0.025.
K. ascorbata SUD165 cells diluted to an absorbance at 600 nm of 0.50.
ND, not determined.