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. 2023 Nov 2;24(21):15920. doi: 10.3390/ijms242115920

Figure 4.

Figure 4

Cucurbitacin I downregulates the expression of the M2-related gene Hmox1 in macrophages from hypoxia or coculture systems. (A) Overlap of DEGs at two intersections, presented as a Venn diagram. (B) A statistical histogram showing several Hmox1-enriched GO and KEGG pathways. (C) Heatmap depicting Hmox1 as being significantly linked with the expression of M2-related genes in 33 TCGA cancer types. (D–H) The mRNA and protein levels of Hmox1 were significantly elevated in macrophages either in coculture systems with MCF7 (the third protein lane) or HCT116 (the fourth protein lane) cells, or under hypoxia (the second protein lane) via qRT-PCR and Western blot analysis. (E–G,I–K) The mRNA and protein levels of Hmox1 were remarkably decreased in macrophages treated with cucurbitacin I either in cocultured systems with HCT116 or MCF7 cells or under hypoxia via qRT-PCR and Western blot analysis. Results for all panels were independently reproduced at least three times. The values presented are expressed as mean ± standard deviation (SD) and are based on data obtained from three biologically independent samples. p < 0.05: *, p < 0.01: **, p < 0.001: ***.