Table 1.
Limit of detection for DNA targets. The limit of detection reported in estimated MAF as determined by ChromaCode Cloud 5.0 software. Input amount is defined in ng for each well as measured by Qubit. Mean counts represent the average number of positive partitions at the reported limit of detection. The detection rate is the positive results out of 20 total replicates.
| 7.5 ng Input | 20 ng Input | |||||
|---|---|---|---|---|---|---|
| Target | Mean Counts | Detection Rate | Estimated MAF | Mean Counts | Detection Rate | Estimated MAF |
| EGFR Exon 19 deletions | 76.7 | 20/20 | 10.9% | 30.2 | 20/20 | 1.2% |
| EGFR G719X | 40.9 | 20/20 | 6.3% | 33.8 | 20/20 | 2.7% |
| EGFR S768I | 45.5 | 20/20 | 9.6% | 63.9 | 20/20 | 3.2% |
| ERBB2 insertions | 41.6 | 20/20 | 7.4% | 32.2 | 20/20 | 2.4% |
| EGFR L858R | 70.8 | 20/20 | 8.8% | 58.4 | 19/20 | 2.6% |
| EGFR Exon 20 insertions | 60.4 | 20/20 | 10.1% | 58.8 | 20/20 | 4.9% |
| BRAF V600E | 21.0 | 20/20 | 2.4% | 44.3 | 20/20 | 2.1% |
| KRAS G12C | 23.1 | 20/20 | 3.6% | 34.1 | 20/20 | 2.3% |
| EGFR T790M | 37.2 | 20/20 | 9.5% | 19.3 | 20/20 | 0.8% |
| EGFR L861Q | 40.4 | 20/20 | 8.3% | 39.9 | 20/20 | 3.4% |