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Detection of DNA amplification in the loop-mediated isothermal amplification (LAMP) assay by a change in color from orange to yellow. LAMP results in addition of SYBR green dye showing A1 and A2 as positive for Aeromonas hydrophila, S1 and S2 as positive for Streptococcus agalactiae, and NC (negative control) as a negative reaction. The LAMP reaction product was analyzed on a 2% agarose gel.
